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Direct Antiglobulin

Does this test have other names?

Direct Coombs test

What is this test?

The direct antiglobulin test is a blood test used to diagnose a type of anemia caused by your immune system. Your immune system is your body's defense system. It makes proteins called antibodies to attack foreign invaders. In some cases, your immune system can make antibodies against red blood cells. This causes red blood cells to break down, a condition called hemolytic anemia.

Why do I need this test?

This blood test tells your healthcare provider whether you have antibodies that have attached to your red blood cells. You may need this test if you have symptoms of hemolytic anemia after a blood transfusion. A baby may need this blood test if the baby's mother makes antibodies against the baby's red blood cells and passes those antibodies to the baby inside the womb. This condition is called hemolytic disease of the newborn.

The most common cause of hemolytic anemia is when your immune system makes antibodies to your own red blood cells by mistake. When your immune system makes antibodies against your own healthy cells, it is called autoimmune disease. Symptoms or signs of hemolytic anemia may include:

  • Tiredness

  • Shortness of breath

  • Headaches or dizziness

  • Coldness in your hands and feet

  • Yellowing of your skin (jaundice) and dark urine

  • Heart problems such as an irregular heartbeat, heart murmur, or heart failure

  • Blood tests that show low numbers of red blood cells 

What other tests might I have along with this test?

You may have a blood test that measures the red blood cells in your blood and the amount of oxygen carried by your red blood cells. These tests are called hemoglobin and hematocrit.

What do my test results mean?

A result for a lab test may be affected by many things, including the method the laboratory uses to do the test. Even if your test results are different from the normal value, you may not have a problem. To learn what the results mean for you, talk with your healthcare provider.

The direct antiglobulin test tells your healthcare provider whether you or your child has antibodies to red blood cells. A normal blood test will find no antibodies to red blood cells. If there are any antibodies to red blood cells, the test is considered positive. The test results may range from 1+ (barely positive) to 4+ (very positive). A positive antiglobulin test may mean:

  • Reaction to a blood transfusion

  • Autoimmune hemolytic anemia

  • Hemolytic disease of the newborn

  • Hemolytic anemia caused by a drug reaction 

How is this test done?

The test requires a blood sample, which is drawn through a needle from a vein in your arm.

Does this test pose any risks?

Taking a blood sample with a needle carries risks that include bleeding, infection, bruising, and a sense of lightheadedness. When the needle is put into your arm, you may feel a slight stinging sensation or pain. Afterward, the site may be sore.

What might affect my test results?

Some medicines can interfere with this test. And some medicines can cause the test to come back positive even though you don't have hemolytic anemia. Medicines that may cause a hemolytic anemia result include:

  • Certain antibiotics

  • Anti-inflammatory medicines

  • Medicines used for malaria

  • Chemotherapy medicines

How do I get ready for this test?

You don't need any special preparations for this test. Make sure to let your healthcare provider know about any medicines, herbs, or supplements you are taking. This includes medicines that don’t need a prescription and any illicit drugs you may use.

Direct Antiglobulin - WellSpan Health

Author: Iliades, Chris, MD
Online Medical Reviewer: Sather, Rita, RN
Online Medical Reviewer: Ziegler, Olivia Walton, MS, PA-C
Last Review Date: 2015-08-15T00:00:00
Last Modified Date: 2015-09-04T00:00:00
Published Date: 2015-09-04T00:00:00
Last Review Date: 2012-04-04T00:00:00
© 2016 WellSpan Health. All Rights Reserved. This information is not intended as a substitute for professional medical care. Always follow your healthcare professional's instructions.

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